• Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR) developed the GQ-RCP diagnostic platform for detecting HIV with high precision.

• It targets the unique G-Quadruplex (GQ) DNA structure in the HIV genome, reducing false positives common in existing diagnostic methods.

• It uses benzobisthiazole-based fluorescent probes (TGS64) for selective and reliable detection via reverse transcription and amplification of a 176-nucleotide sequence. 

• Featuring a pH-mediated single-step DNA transition into GQ conformation, enabling efficient recognition.

• This new method offers significant advantages over conventional diagnostics by mitigating non-specific amplification issues.

• Originally designed for pathogens like SARS-CoV-2, the platform is adaptable for diagnosing various DNA/RNA pathogens, including bacteria and viruses.

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